《植物生理学报》 2014, 50(4): 527-534

铁核桃离体培养与快速繁殖

王沙沙^1,2, 潘学军^1,2,*, 张文娥²

¹贵州省果树工程技术研究中心, 贵阳550025; ²贵州大学农学院, 贵阳550025

通信作者：潘学军；E-mail: pxjun2050@aliyun.com；Tel: 0851-3855894

摘　要：

以优良单株‘纳雍-1’的单芽茎段为外植体, 建立了铁核桃(Juglans sigillata)离体培养与快速繁殖的体系。结果表明, 附加6-BA 1.0 mg·L^-1+活性炭(AC) 3.0 g·L^-1的DKW培养基适宜铁核桃腋芽诱导; 适宜铁核桃芽增殖的培养基为DKW+6-BA 1.0 mg·L^-1+IBA 0.02 mg·L^-1, 40 d后增殖系数可达7.33; 试管苗的茎尖和茎段均可用于增殖培养; 一步生根法(低浓度的生长素IBA持续诱导)不利于铁核桃试管苗嫩茎生根; 采用二步生根法, 生根率最高可达71.73%, 其中, 不同IBA浓度、暗培养时间、蔗糖浓度和AC含量对试管苗嫩茎生根影响显著, 铁核桃试管苗在附加IBA 5.0 mg·L^-1的1/4DKW培养基中暗培养12 d, 再转移到不含IBA的1/4DKW培养基(附加AC 3 g·L^-1和蔗糖20 g·L^-1)中生根效果最好; 生根试管苗采用珍珠岩和营养土两步炼苗, 60 d后成活率达到87.50%。

关键词：铁核桃; 单芽茎段; 离体培养; 快速繁殖; 生根

收稿：2014-01-16   修定：2014-01-29

资助：贵州省科技攻关项目(NY20073038)、贵州省科技重大专项子课题(20116011)和贵州大学研究生创新基金项目(2013008)。

In Vitro Culture and Rapid Propagation of Juglans sigillata

WANG Sha-Sha^1,2, PAN Xue-Jun^1,2,*, ZHANG Wen-E²

¹Guizhou Engineering Research Center for Fruit Crops, Guiyang 550025, China; ²Agricultural College of Guizhou University, Guiyang 550025, China

Corresponding author: PAN Xue-Jun; E-mail: pxjun2050@aliyun.com; Tel: 0851-3855894

Abstract:

Single bud stem of superior individual Juglans sigillata cv. ‘Nayong-1’ were used as explants to establish in vitro culture and rapid propagation system. Results showed that the DKW medium supplemented with 1.0 mg·L-1 6-BA+3.0 g·L-1 AC was suitable for germination of axillary buds. The DKW medium with 1.0 mg·L-1 6-BA+0.02 mg·L-1 IBA was optimal for the shoots multiplication, the proliferation coefficient was up to 7.33 after culturing for 40 days. Both shoot tip and single axillary bud could be used for multiplication culture. One step method (low IBA concentration sustained induction) was not suitable for rooting culture. The highest rooting rate (71.73%) was obtained by two steps method, firstly the plantlets was cultured on 1/4DKW medium with 5.0 mg·L-1 IBA in the dark condition for 12 days, and then transferred to the 1/4DKW medium without IBA under light (3 g·L-1 AC and 20 g·L-1 sucrose). It was found that the IBA concentrations, time of dark induction, sucrose concentrations and AC contents were all related to the effect of rooting. After acclimation in pearlite and nutrient soil for 60 days, 87.50% plantlets were survival.

Key words: Juglans sigillata; single bud stem; in vitro culture; rapid propagation; rooting